美国SKC采样器Biosampler样品采集与分析可适用于以下几种分析法:
生产培养法菌落分析
用生长培养法定量分析和表征空气培养细菌和真菌。
液体样品的一部分被转移到这些微生物是长成菌落计数培养基。
微观分析
显微镜分析用于计数和有限的鉴定空气中的细菌和真菌总数,生物颗粒是区别于非生物染色技术。
生化测定
生化法是用来测量如内毒素生物化合物。一种化学物质或生物物质被加到样品的一部分。随后的反应形成定量的基础。
免疫分析法
免疫分析是用于空气中的过敏原定量(如尘螨、动物皮屑)。它依赖于抗体与特定靶抗原的结合。其他方法包括荧光、酶和放射免疫分析。
聚合酶链反应
聚合酶链反应(PCR)用于筛选特定属或种的样本。PCR过程是基于对选定核酸序列的体外复制。
SKC BioSampler是一个具有(Patented 5,902,385)的率玻璃冲击瓶捕集装置的特殊设计,它需要一台高流量音速的空气采样泵, 用来捕集空气中微生物(如细菌、真菌、花粉、病毒)与其副产物如内毒素、霉菌毒素及其它碎片,以供进一步分析。
analysis
samples collected with the biosampler can be analyzed using:
Growth Culture Analysis
Growth culture analysis is used to quantify and characterize airborne cultural bacteria and fungi.
A portion of the liquid sample is transferred to a culture medium on whic the microorganisms are grown into colonies for counting.
A broad-spectrum of media is gennerally used.
Microscopic Analysis
Microscopic analysis is used for used for the enumeration and limited identification of total airborne bacteria and fungi,Biological particles are differentiated from nonbiological ones by staining techniques.
Biochemical Assay
Biochemical assay is used to measure bilogical compounds such as endotoxins. A chemical or biological substance is added to a portion of the sample
the ensuing reaction forms the basis for quantification
immunoassay
immunoassay is used for the quantification of airborne allergens(e.g.,dust mites and animal dander).
It relies on the binding of antibodies to a specific target antigen. Other methods include fluorescence,enzyme,and radioimmunoassay.
Polymerase Chain Reaction
Polymerase Chain Reaction(pcr) is used to screen samples for a specific genus or species.
the PCR process is based on in-vitro replication of selected nucleic acid sequences.